Fetal Sex Determination Using Cell-Free Fetal Dna (cffDNA) in Maternal Blood

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Prenatal determination of farm animal fetal sex using free fetal DNA in maternal plasma

In many farm animal species, the preference of a specific sex is obvious mainly due to commercial implications; so for comprehensive and efficient results in the livestock management plans specially breedingand selection programs, it is necessary to include acost-effective and reliable strategy for early determination of fetal sex. Several invasive and non-invasive approaches have been establis...

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Evaluation of Sample Stability and Automated DNA Extraction for Fetal Sex Determination Using Cell-Free Fetal DNA in Maternal Plasma

OBJECTIVE The detection of paternally inherited sequences in maternal plasma, such as the SRY gene for fetal sexing or RHD for fetal blood group genotyping, is becoming part of daily routine in diagnostic laboratories. Due to the low percentage of fetal DNA, it is crucial to ensure sample stability and the efficiency of DNA extraction. We evaluated blood stability at 4°C for at least 24 hours a...

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highly sensitive conventional pcr testing of free fetal dna in maternal serum for non-invasive fetal sex determination

background: free fetal dna (ffd) in maternal plasma/serum has increasingly become the source of fetal material for diagnostic purposes in recent years. this source of fetal material can be used for sex determination. rh typing paternally inherited sequences and compound heterozygosity. reports on the lack of consistent pcr amplification of y-chromosome sequences of ffd in maternal plasma/serum ...

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early non-invasive determination of fetal sex using cell-free dna

the first step in the prenatal diagnosis of x-linked genetic disorders is determining fetus gender. current invasive methods to obtain the dna source of the fetus instead of its miscarriage risk, has harmful stress for high risk pregnancies. cell free fetal dna (cffdna) circulating in the maternal blood, has now become a useful source of noninvasive prenatal diagnosis. considering limitation of...

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Critically Appraised Topic Fetal RHD genotyping in maternal blood using cell-free fetal DNA

The discovery of cell-free fetal DNA (cffDNA) in maternal plasma has opened up new possibilities for non-invasive prenatal diagnosis (NIPD). Real-time PCR protocols as well as MALDI-TOF mass spectrometry techniques have been developed to determine fetal RHD genotype on maternal plasma. In several European centers, NIPD for fetal RHD has become the standard of care for evaluation of anti-D allo-...

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ژورنال

عنوان ژورنال: Bali Medical Journal

سال: 2016

ISSN: 2302-2914,2089-1180

DOI: 10.15562/bmj.v5i2.197